AS the part of high-throughput capabilities at HDB, RapidFire high throughput mass spec system supports our clients on in vitro ADME assays, cancer metabolite analysis, and other mass spec based high–throughput

RapidFire 360 (Agilent Technologies) combines the accurate analytical capabilities of mass spectrometry and high-throughput processing speed. It eliminates major steps in sample preparation and method development associated with conventional approaches and streamlines the work flow. The samples can be delivered to the

Broad utilization in in vitro ADME assays with outstanding throughput,

•  MRM-based targeted metabolite screening platform to support target validation and

extremely short cycle time, and possible elimination of method optimization

    other exploratory study in cancer metabolism

to achieve the most efficient work flow

•  Targeted metabolites cover various cell metabolism pathways (Glycolysis,

•  Metabolic stability (microsomes and S9)

    Glutaminolysis,TCA, GSH, etc.)

•  CYP inhibition (RI ,TDI)

•  Compatible with various biological matrices (cell lysate/plasma/tissue)

•  Protein/tissue binding and blood partitioning

•  Method application in selected cancer cell lines

•  Solubility/stability

•  Permeability (PAMPA, Caco-2, MDCKII)

•  Customized biochem or cell-based RF/MS assay development

•  Capable of screening of intractable or challenging targets

•  10x faster throughput than conventional MS screening methods