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Liver Diseases
Acute and chronic liver diseases are frequent and potentially life threatening for humans. The underlying etiologies are diverse, ranging from viral infections, autoimmune disorders, and intoxications to imbalanced diets and others. Therefore, animal liver disease models are in needs to mimic human liver diseases. HD Biosciences has established multiple animal liver disease models including acute liver injury; obesity related liver damage; nonalcoholic steatohepatitis (NASH); alcoholic steatohepatitis (ASH), liver fibrosis, liver cirrhosis and hepatocellular carcinoma (HCC). These models posse distinct features and etiology of human liver diseases to help evaluate the efficacy of lead compounds in relevant clinical liver diseases and are designed for better understanding of underlying.
mechanisms for new drugs to be used in clinic
In vitro biochemistry, cell-based and functional assays
MOA and functional analysis including evaluation of cell proliferation, cell apoptosis and
specific fibrogenic pathways
Characterization of markers involving in liver injury, fibrogenesis or fibrolysis at gene and
protein levels with qPCR, ELISA, western blot, LC/MS/MS, etc.
Human HSC LX-2, rat HSC T6, primary rodent HSC and hepatocyte systems
In vivo animal models
Acute liver injury models
Alcohol induced acute liver injury in Kunming mouse & non human primate (NHP)
CCl4 induced acute liver injury in rodents & NHP
Chronic fatty liver models
Obesity in liver damage rodent models
HFD-induced fatty liver in mice and rats
ob/ob mice, db/db mice with fatty liver
Obesity and liver damage in NHP model
HFD-induced fatty liver in cynomolgus monkeys
Chronic liver injury models
Liver fibrosis/cirrhosis models
CCl4 induced liver fibrosis (mice, rats)
CCl4 induced liver cirrhosis (mice, rats)
Bile duct ligation (BDL) induced liver fibrosis (mice, rats)
Fatty liver + CCl4 induced liver fibrosis (mice, rats, NHP)
Fatty liver + CCl4 induced liver cirrhosis (mice, rats)
Alcohol induced chronic liver damage (NHP)
Non Alcoholic steatohepatitis (NASH) / HCC models
Fatty liver with NASH model
DIO + CCl4 induced NASH in mice
DIO + CCl4 induced NASH in NHP
STZ + HFD induced NASH, fibrosis, cirrhosis and HCC in mice
Complicated factor induced chronic liver injury model
High fat diet+alcohol+CCl4 induced liver fibrosis in monkey model
Endpoint Measurement
Clinical observation and physical examination
Body weight, behavior, food intake, water intake, defecation
Liver weight and spleen weight, ascite measurement, portal vein pressure
Biomarker analysis
Serum ALT / AST analysis
Gene expression and profiling (RT-PCR, microarray gene expression, miRNA)
Protein and metabolite profiling (WB, ELISA, mass spectrometry)
Hepatic hydroxyproline detection
Histology analysis
H&E staining in liver tissues
Sirius red staining and Masson Trchrome staining for collagen deposition and quantification in liver tissue
α-SMA IHC staining and quantification in liver tissues
Other related IHC staining for focused targets and markers
Representative Data
Liver damage in obese mouse models
Fatty liver in obese mice
Animal: C57BL/6J, male, 4-6 weeks of age; Diet: high fat diet (HFD, D12492, 60 kcal%, Research Diets, Inc) over 16 weeks. Body weight is about 40 gram. Severe fatty liver is observed.
Liver fibrosis in obese mice (NASH)
Animal: C57BL/6J, HFD 20 weeks, CCl4 for 4 weeks. H&E: Hepatocyte balloon degeneration with portal area inflammatory cell infiltration. Sirius Red: Collagen deposition in portal and intra lobular area. PAS: Glycogen deposit in hepatocytes.
CCl4 induced chronic liver damage in mouse and rat models
CCl4 induced liver fibrosis in mice
Balb/c mice, CCl4 0.5ul/g (1:5) i.p. twice a week for 8 weeks. Blood serum ALT and AST elevated significantly. Liver collagen deposition and myofibroblast proliferation have been detected.
CCl4 induced liver cirrhosis in rats
SD rats, CCl4 1.5ul/g (1:1) o.p. twice a week for 8 weeks. Histologically, inflammatory cell infiltration, hepatocyte degeneration, fibrosis and cirrhosis were observed.
HD Biosciences (China) Co.,Ltd
590 Ruiqing Road
Zhangjiang East Campus, Pudong, Shanghai 201201,
Tel: +86 (21) 5116 3700
Fax: +86 (21) 5116 3766
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Pudong, Shanghai, 201201 San Diego, CA, 92121 Cranbury, NJ 08512
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Tel: +86 (21) 5116-3700 Tel: +1 (858) 888-7888 Tel: +1 (609) 606-6680
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