HD Biosciences Advanced Gene Manipulation (AGM™) technology and platform provide comprehensive solutions to our clients for their gene manipulations of knock-down, knock-out, knock-in and over-expression, so to understand the gene of interest in functional impact and mechanism of actions. Various types of genetic manipulation including AAVs-based vectors, proprietary genome editing, and transgenic technology offer cell models and animal models with region specific, tissue specific and cell type specific, in supporting target

Integration of AGM™ with in vitro, in vivo pharmacology, biomarkers, and bioinformatics enables HD Biosciences to grow into a leader in the services around target discovery, validation, translational sciences and

Comprehensive viral-based & non-viral-based vectors in specific cells,

Targeted genome editing platform for knockout, knock-in at cell types of

tissue, organ or systemic gene delivery & manipulation in vitro & in vivo

interest

•  AAVs-based (various serotypes) on efficient & less tox manipulation &

•  Reporter-based assay systems using endogenous or external promoters

    delivery

•  Modification of endogenous genes (e.g. polymorphisms, site-directed

•  Lentiviral-based mediated inducible cell lines for xenograft models

    mutations)

•  Retroviral-based gene manipulation in cell lines

•  Biologic function of any gene or pathway

•  siRNA & shRNA-based gene manipulation

•  Physiologically relevant genes, Boosting or retaining

•  siRNA and CRISPR sgRNA library screening and target id

•  CRISPR/cas9 technology for isogenic cell line generation

Transgenic technology for the generation of animal disease models &

•  In vitro functional readouts, gene expression profiling, target MOA, &

target engagement for proof-of-concept

    pathway analysis

•  Conventional knockout, knockdown, & knock-in mice

•  In vivo phenotypic characterizations in various disease models

•  Conditional, brain, other organ, or cell type specific knockout mice

    (Metabolic disease, Oncology, CNS, & inflammation)

•  Region or cell type specific inducible/reversible transgenic mice

•  Biomarker discovery, IHC, in situ protein expression with bioinformatics supports

•  BAC/YAC transgenic mice for humanized animal model

•  Bioinformatics supports & data analysis