|
|
|
|
|
Transgenic Services |
|
|
|
HDB Functional Genetic Center (HDB-FTC) is a custom request-based platform providing full-scale service from gene cloning, making gene expression vector/targeting construct, pronuclear/blastocyst microinjection, genotyping, to characterizations of phenotypes at both in vitro and in vivo levels in the mouse, among which the last capacity/feasibility |
differentiates HDB-FTC from other transgenic resources. |
|
|
|
|
|
Fig. 1. Generation of knockout mice by homologous recombination. A. Chimeric mice between 129/SEV ES cells (agouti) and C57BL6 (J6) blastocysts (black). B. Potential germline transmission of their pups with agouti fur color. |
• |
Making expression vector for producing transgenic mice, making gene targeting construct for producing knockout, knockdown, and knock-in mice, and constructing BAC clones containing haplotype(s) or vulnerable loci for a particular type of disease for generating
|
|
BAC transgenic mice |
|
|
|
|
• |
Homologous recombination, ES cell screening, determining transgene copy number in transgenic founder mice |
|
• |
Mouse breeding, mouse genotyping, and determining heterozygous/homozygous in |
|
knockout/down/in mice, and hemizygous/homozygous in transgenic mice |
|
• |
Basic characterization of mutant mice at molecular and histological levels |
|
|
|
• |
Phenotypical characterization of mutant mice in various systems including behaviors, cancer biology and oncogene, cardiovascular system, CNS, immunology, inflammation and infection, metabolic |
|
 |
|
disease, and other systems |
|
|
Types of genetic manipulations |
|
|
• |
Common (conventional) knockout, knockdown, and knock-in mice |
|
Fig. 2. BDNF knockdown mice. An obese
phenotype is observed in knockdown mice (left), compared to wild-type mice (right). |
• |
Common transgenic mice |
|
|
|
• |
Conditional, brain region-specific, organ-specific, and cell-type-specific knockout mice |
|
• |
Brain region-specific and cell type-specific inducible/reversible transgenic mice |
|
• |
BAC/YAC transgenic mice |
|
• |
Small RNA manipulation |
|
• |
Zinc finger nuclease in rat and cell lines (under development) |
|
|
|
|
Fig. 3. Inducible/reversible forebrain-specific
transgenic mice. A. wild-type mice-treated with vehicle.
B. transgenic mice-treated with vehicle. C. Transgenic
mice mice-treated with doxy for 3 days. D. Transgenic
mice-withdrawn doxy for 5 days after 3-day-doxy
treatment. |
Fig. 4. Cell type-specific expression of a
reporter gene (Lac-Z). A. Cerebellum-specific. B.
Cerebellar cortex-specific. C. Cerebellar Purkinje’s
cell-specific. D. Cerebellar granule cell-specific |
|
|
|
|
|
|
|
|
|
 |
|
|
The Latest Generation of Auto Patch Clamp Platform Launched at HDB  |
|
---------------------- |
WuXi Biology/HD Biosciences Presented at Webinar of "New Technologies for Target Discovery and Validation" |
|
---------------------- |
HD Biosciences Merges with WuXi AppTec |
|
|
|
|
|
|
|
|
|
|
沪公网安备 31011502003320号