The deficiency in energy expenditure is an important etiological determinant and also the hallmark for metabolic diseases. Oxymas/CLAMS system provides 24-hour non-invasive collection of locomotor activity, feeding, drinking, urine, and indirect calorimetry data including volume of oxygen consumed, volume of carbon dioxide generated and the respiratory exchange ratio. The energy expenditure analysis predicts the animal metabolic status and therapeutic effects of test compounds. HDB has set up the Oxymas configuration systems with positive drug validation and compound evaluation on several physiological and behavioral parameters in both normal lean and high-fat diet induced obesity (DIO) mice.