| Dermatology in vitro Assays |
|
• |
|
Melanin synthesis with the co-culture of Nornal Human |
|
Keratinocytes (NHK) and Melanocytes (NHM) |
• |
Keratinocyte assays with conditioned PBMC medium |
• |
Oxidative stress assays on Human Dermal Fibroblasts |
• |
Normal Human Epidermal Keratinocytes (NHEK) qPCR |
|
study |
• |
Normal Human Epidermal Keratinocytes (NHEK) |
|
proliferation assay |
|
|
| Dermatology in vivo Models |
| Atopic Dermatitis (AD) |
Contact hypersensitivity (CHS) |
• |
|
Tofacitinib Effects in OXA Induced AD in |
• |
|
FITC induced CHS in Balb/c mice |
|
Balb/c Mice |
|
• |
Temovate Effects in DNFB Induced AD in |
Psoriasis |
|
Balb/c Mice |
• |
|
Imiquimod induced psoriasis in Balb/c |
• |
DNCB Induced AD in Balb/c Mice |
|
mice |
• |
House Dust Mite (DFE) Induced AD in |
• |
IL-23 Induced Psoriasis-like Inflammation |
|
Balb/c Mice |
|
in Balb/c mice |
• |
PMA induced AD/ear edema in |
|
|
male/female mice |
ITCH Models |
|
• |
|
AEW/Chloroquine induced ITCH in |
| Delayed Type Hypersensitivity (DTH) |
|
C57BL/6 mice |
• |
|
Rodents (mice and rats) |
• |
Histamine induced ITCH in C57BL/6 mice |
• |
|
OXA induced DTH in Balb/c mice/Wistar |
|
|
|
Rats |
Histamine Wheal Model |
• |
|
DNFB induced DTH in Balc/c mice |
|
• |
|
KLH induced DTH in Balb/c mice |
• |
|
mBSA induced DTH in C57BL/6J mice |
|
|
|
|
| Representative in vitro Assays |
Case 1: Melanin Synthesis Assay using Normal Human Keratinocytes (NHK) and Melanocytes (NHM) |
|
| The NHK-NHM co-culture screening assay provides a useful tool to compare the activity of known modulators of melanogenesis and to perform structure-activity studies with newly |
| identified modulators to improve their activity. |
|
 |
|
|
| Changes in the rate of melanin synthesis will be measured on the basis of 14C-thiouracil incorporation into newly synthesized melanin. |
| Changes in 3H-leucine incorporation will be taken as an indication of cytotoxicity or induction of proliferation. |
|
|
|
|
|
|
Case 2: Keratinocytes qPCR study |
|
| To identify suitable NHEK functional assay readout as measurement of compound effect on |
| keratinocyte and skin barrier function |
|
 |
|
|
|
| Expression levels of several genes in normal human epidermal keratinocyte (NHEK) increased after PMA |
| treatment for 48 hours. |
|
|
|
|
|
| Representative in vivo Case Studies |
Case 1: Tofacitinib Effects in Oxa Induced Atopic Dermatitis Mice Model |
 |
|
| Sensitization: 10ul of 5% Oxa in acetone/olive |
| oil (4/1) on back skin of animal. |
| Challenging: mice were treated topically with 100ul of 0.1% Oxa in acetone/olive oil (4/1) |
| on back flanks (once every two days). |
|
|
 |
| Note: Tofacitinib significantly decreased OXA induced skin thickness increase, skin TNFα |
| expression increase and skin pathology composite score and epidermal thickness increase |
|
|
|
Case 2: Compound Effects in DNFB Induced Atopic Dermatitis Mice Model |
 |
 |
|
| 1. |
|
DNFB induced AD model were well established in Balb/c mice |
| 2. |
Temovate significantly decreased DNFB induced ear thickness and weight increase and |
|
skin pathology composite score increase and pidermal thickness increase |
| 3. |
We collaborated with several companies for efficacy and MOA studies. |
|
|
|
|
Case 3: Compound Efficacy in DNCB Induced Atopic Dermatitis Mice Model |
|
 |
|
| 1. |
|
DNCB induced atopic dermatitis model was well established at HDB |
| 2. |
CompX significantly decreased DNCB induced skin thickness and clinical score increase |
| 3. |
We collaborated with several companies for efficacy studies. |
|
|
|
|
Case 4: Compound Effects in House Dust Mite (DFE) Induced Atopic Dermatitis Mice Model |
|
 |
|
| 1. |
|
House dust mite and DNCB induced atopic dermatitis model was successfully |
|
established at HDB |
| 2. |
Dex at 1mpk p.o/qd significantly decrease house dust mite and DNCB induce ear |
|
thickness and dermatitis score increase |
| 3. |
Dex at 1mpk p.o/qd significantly decrease house dust mite and DNCB induced ear |
|
TNFa/IL-13 gene expression increase |
| 4. |
Several on-going studies for several clients |
|
|
 Connect with us for more information |
|
沪公网安备 31011502003320号