CRISPR/Cas9 genome editing system becomes a powerful tool to target and modify genomic sequence, allowing a previously unattainable level of efficacy, specificity and efficiency. This system uses non-coding RNAs to guide the Cas9 nuclease to generate sequence-specific DNA cleavage of target loci across the genome. HD Biosciences has fully implemented this technology platform to offer CRISPR/Cas9 based gene knock-in or |
knock-out to support gene function studies and target validation efforts. |
|
|
|
CRISPR/Cas9 Applications |
|
• |
|
Specific gene knock-out |
• |
Precise genome editing, such as site-mutation knock-in or add reporter gene tag |
• |
Rapid generation of genetically engineered mice |
• |
Genome-wide scale knock-out for screening |
|
|
Key Advantages of CRISPR/Cas9 |
|
• |
|
Simple and fast two-component system with high efficiency and low cost |
• |
Permanently turn off genes at the DNA level, clean background |
• |
Offers access to non-coding regions of the genome |
|
|
HDB CRISPR/Cas9 Services |
|
• |
|
sgRNA design, plasmid construction, and validation |
• |
Knock-out of single or multiple target sequences |
• |
Site-mutation knock-in and gene tag knock-in |
• |
Isogenic cell line generation with gene knock-out/knock-in |
• |
Genome-wide CRISPR/Cas9 library screen |
|
|
Case study 1: Knock-out of EGFP expression |
|
|
|
U2OS cells with stable EGFP expression(U2OS_EGFP) were transfected with lentiCRISPR vectors containing Cas9 and either scramble sgRNA or sgRNA specifically targeting EGFP. |
|
Case study 2: Target X Knock-out - CRISPR plasmid transfection |
|
|
|
Tumor cells were transfected with vectors containing Cas9 and either scramble sgRNA or sgRNA specifically targeting Target X in order to generate isogenic Target X KO cell lines. |
|
Case study 3: Target Y Knock-out - LentiCRISPR transduction |
|
|
|
Tumor cells were transduced with LentiCRISPR vector containing Cas9 and either scramble sgRNA or sgRNA specifically targeting Target Y in order to generate isogenic Target Y KO |
cell lines. |
|
Flow chart for isogenic cell line generation |
|
|
|
沪公网安备 31011502003320号