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Anti-bacteria Program |
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With an experienced team, fully equipped BSL-2 microbiology labs, HDB has built up comprehensive capabilities and capacities to support antibacterial drug discovery. HDB has the extensive collections of characterized bacterial strains, varieties of assays and |
matching screening capability. |
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| Bacterial Strain Collection |
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Over 50 standard QC strains and over 900 clinical isolates |
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Strain identification through 16S rRNA sequence, VITEK2 or MALDI-TOF MS analysis |
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AST profile of 16 commercial antibiotics |
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Drug resistant gene identification through multiplex PCR analysis |
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| Antibacterial Susceptibility Testing |
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Minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC) |
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MIC50 / MIC90 |
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MITC, tMBC |
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| Kinetics Study |
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Time-kill study |
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Post-antibiotic-effect (PAE) study |
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| Resistance Test |
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Frequency-of-resistance (FOR) assay |
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Resistance mutant gene identification |
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| Synergy Test |
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Checkerboard assay |
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E-test method |
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| MOA Study |
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Macromolecule synthesis (MMS) assay - Macromolecular synthesis pathways including |
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DNA, RNA, protein, phospholipid, peptidoglycan and lipopolysaccharide synthesis are examined by radioactivity quantitation of newly synthesized macromolecules that are |
| radiolabeled using different radioisotope substrates.
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Transcription and translation (TNT) assay is to determine the effect of test compounds |
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on RNA transcription and/or protein translation in a cell-free system. |
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Bacterial lysis assay - E. coli cell lysis assay is to quantify activity of extracellular |
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heterogeneous β-galactosidase released during cell lysis because of cell wall and cell |
| membrane damage. |
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Bacterial membrane depolarization assay is to measure bacterial cell membrane |
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potential changes in presence and absence of compounds using potential-sensitive |
| fluorescent dye. |
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| Target Specific Assays |
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E. coli and S. aureus gyrase and topo IV |
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study |
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Β-lactamase and metallo- Β-lactamase |
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inhibition assay |
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CoaBC enzyme inhibition assay |
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Dihydrofolate reductase assay |
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Pseudomonas pyocyanin assay |
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| Other Assays |
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Hemolysis assay |
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Serum shift assay |
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Nephrotoxicity assay |
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E. coli gene knockout using CRISPR-Cas9 |
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system |
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Bacterial genomic analysis and |
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phenotypic study |
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