As a relatively simple but extremely powerful tool for studying receptors and ion channels, radioligand binding assay is widely employed to analyze interactions of hormones, neurotransmitters, grow factors and related drugs with the receptors/ion channels. At HDB we built the capacity of performing HTS-compatible large scale radioligand binding assay screening for all the GPCRs and specific ion channels from our cell lines list at low costs. We have optimized protocols for both ligand binding assays such as hERG binding and GTPgammas binding such as opioid receptors and group II and III mGluRs. Moreover with advanced Toshiba high-speed refrigerated centrifuges and IKA homogenizers, we built strong membrane preparation capacity targeting numerous GPCRs at a much lower price compared to other vendors. The radiometric assays can also be used in kinase assays using 32P-ATP or PDE assays using 3H-cAMP or 3H-cGMP.