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Toxicology
HDB is committed to providing in vitro and in vivo toxicology services which support the early drug discovery and lead selection/optimization. In continuation of plate-based efficacy services, HDB provides rapid and valuable safety evaluation to define the potential of new compounds in your pipeline. Client's compounds will be quickly analyzed for the safety profile in
early stage through most our in vitro HTS in vitro toxicology assay as low as 10ul of 10mM (eg, ~10ul of 10 mM compound can be used for most following in vitro toxicology assays). For further evaluation of compound toxicity, in vivo toxicology study can be initiated and
performed in rodents soon in our new SFP level IACUC animal facility.
In vitro toxicology
We provide robust and rapid in vitro toxicity screening approaches to identify compound liabilities and its STR (structure toxicity relationship) in order to further optimize NCE in an early drug discovery stage. Our fast turnaround results from multiple assays and dose-response profiles help our clients to define the potential toxicity of compounds and assist our clients to select lead compounds with higher probability of success and faster speed. Most of our assays are in high throughput format that requires small amounts of test
compound with fast data turnaround.
Cytotoxicity
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CellTiter Glo ATP depletion assay
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LDH assay
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MTT assay
Assays are available in HepG2 cells, THLE cells, other hepatocyte, astrocytes, neuron
cells and others upon client request.
Cardiotoxicity
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hERG patch clamp and binding assays
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Nav1.5 and Cav1.2 patch clamp assay in primary cardiomyocytes
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Cardiomyocytes Beating Measurement Using Label-free and Real-time RTCA Cardio
System
Hepatotoxicity
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Hepatocyte cell viability
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Steatosis and phospholipidosis
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Enzyme induction
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BSEP Assay
Mitochondrial toxicity
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Isolated rat liver mitochondrial (RST/OCR) assay
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Mitochondrial membrane potential assay
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Metabolism (celluar energy flux assay)
Genotoxicity
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Ames test (mini and standard Ames)
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Micronucleus test (CHO/K1)
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Chromosome aberration assay (CHO/K1)
Drug-drug interaction
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CYP induction assay
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P-gp inhibition assay in Caco-2 or MDCK cells
In vivo toxicology studies
HDB in vivo toxicology services provide flexible access to rodents and non-rodent animal species with a variety of administration routes to characterize adverse effects, determine severity and reversibility, to establish a dose-response relationship, to assist in dose selection for clinical trials and to predict genotoxicity. Clinical observation, histopathology, gross pathology, clinical chemistry, hematology and urinalysis will be evaluated in the following study upon your request. Animal facility is SPF level and AAALAC-compliant for
rodent studies.
Local tolerance test
Dose-range-finding study
Acute toxicity study
Repeat dose study
NOAEL (no observable adverse effect level) determination
In vivo micronucleus testing
Toxicokinetics
Related Services
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HD Biosciences (China) Co.,Ltd
590 Ruiqing Road
Zhangjiang East Campus, Pudong, Shanghai 201201,
P.R.China
Tel: +86 (21) 5116 3700
Fax: +86 (21) 5116 3766
info@hdbiosciences.com
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