Advanced Gene Manipulations (AGM™)
HD Biosciences Advanced Gene Manipulation (AGM™) technology and platform provide comprehensive solutions to our clients for their gene manipulations of knock-down, knock-out, knock-in and over-expression, so to understand the gene of interest in functional impact and mechanism of actions. Various types of genetic manipulation including AAVs-based vectors, proprietary genome editing, and transgenic technology offer cell models and animal models with region specific, tissue specific and cell type specific, in supporting target
discovery and target engagement in the context of diseases and the impact of drug treatment.
Integration of AGM™ with in vitro, in vivo pharmacology, biomarkers, and bioinformatics enables HD Biosciences to grow into a leader in the services around target discovery, validation, translational sciences and
lead discoveries and a CRO that can provide inputs to our clients.
Vector Mediated Gene Delivery & Manipulation
Genome Editing & Cell Line Generation
Comprehensive viral-based & non-viral-based vectors in specific cells,
Targeted genome editing platform for knockout, knock-in at cell types of
tissue, organ or systemic gene delivery & manipulation in vitro & in vivo
  AAVs-based (various serotypes) on efficient & less tox manipulation &
  Reporter-based assay systems using endogenous or external promoters
  Modification of endogenous genes (e.g. polymorphisms, site-directed
  Lentiviral-based mediated inducible cell lines for xenograft models
  Retroviral-based gene manipulation in cell lines
  Biologic function of any gene or pathway
  siRNA & shRNA-based gene manipulation
  Physiologically relevant genes, Boosting or retaining
  siRNA and CRISPR sgRNA library screening and target id
  CRISPR/cas9 technology for isogenic cell line generation
Phenotypic Characterization
Transgenic technology for the generation of animal disease models &
  In vitro functional readouts, gene expression profiling, target MOA, &
target engagement for proof-of-concept
    pathway analysis
  Conventional knockout, knockdown, & knock-in mice
  In vivo phenotypic characterizations in various disease models
  Conditional, brain, other organ, or cell type specific knockout mice
    (Metabolic disease, Oncology, CNS, & inflammation)
  Region or cell type specific inducible/reversible transgenic mice
  Biomarker discovery, IHC, in situ protein expression with bioinformatics supports
  BAC/YAC transgenic mice for humanized animal model
  Bioinformatics supports & data analysis