Print E-mail Contact us
PDE and Other Enzyme Assays
In addition to the kinase solution and epigenetics assays, HDB also provides high-quality services including assay development and screening against enzyme targets including but not limited to phosphodiesterases (PDEs), protease, ATPase, integrase, polymerase, ubiquitinase, deubiquitinase and dehydrogenase.
deubiquitinase and dehydrogenase.
Biochemical assay for PDE inhibitors screening
As the important regulators of signal transduction mediated by second messenger molecules such as cAMP and cGMP, PDEs are often targets for pharmacological inhibition due to their unique tissue distribution, structural properties, and functional properties. Applying Perkin Elmers LabChip platform, HDB provides screening and profiling services
against a PDE panel of 17 human PDEs.
HDB PDE Panel
PDE1A
PDE4D
PDE1B
PDE5A
PDE1C
PDE7A
PDE2A
PDE8A
PDE3A
PDE8B
PDE3B
PDE9A
PDE4A
PDE10A
PDE4B
PDE11A
PDE4C
 
IC50 determination of dipyridamole against
several PDEs using Perkin Elmer's LabChip Platform
Cell-based assay for PDE inhibitors screening
cAMP PDEs are emerging as a promising class of drug targets in asthma and cardiovascular disease therapeutic areas. HDB has established the cell-based screening assay for cAMP phosphodiesterase (PDE) inhibitors in HDB based on the Codex ACTOne™ technology. The specificity of the assay has been verified by known PDE inhibitors. Only PDE4 and pan-PDE inhibitors showed positive signals in the cell line that was optimized for
PDE4 inhibitor screening.
Dose response curve of Ro20-1724 and Rolipram generated Prism (Graphpad)
Other Enzymes
We provide custom biochemical or cell-based assay development for other enzymes that
are main therapeutic targets.
Data show consistent IC50 value of one reference compound in Protease A biochemical assay
SPA based assay was established to evaluate one RNA polymerase activity
----------------------
----------------------
HD Biosciences (China) Co.,Ltd
590 Ruiqing Road
Zhangjiang East Campus, Pudong, Shanghai 201201,
P.R.China
Tel: +86 (21) 5116 3700
Fax: +86 (21) 5116 3766
info@hdbiosciences.com
SERVICES
|
THERAPEUTIC AREAS
|
NEWS & EVENTS