Be
Sure to working with Mycoplasma-free cell cultures. |
|
| Defeat
the hidden enemy of cell biologists with the MycoScanTM Mycoplasma Detection Kit |
| Mycoplasma is frequently
found as a contaminant in cell cultures. Studies indicate
that more than 15% of cultures are contaminated. Mycoplasma
can produce a variety of effects on cultured cells
(e.g. changes in metabolism, immunological or biochemical
properties, growth, viability, etc.). It is hard to
detect and also difficult to eradicate, passing easily
through standard filters, and is resistant to most
antibiotics. |
|
| The cost
of a mycoplasma infection could be enormous |
|
| Regular testing for mycoplasma
is the only way to be sure of working with mycoplasma-free
cell cultures, resulting in confidence in the quality
and validity of data obtained. But testing methods
are often cumbersome and time-consuming, or do not
have sufficient sensitivity and specificity. |
|
| Until now |
| HD BIOSCIENCES is proud to introduce
the MycoScanTM Mycoplasma
Detection Kit, a PCR* based assay, which takes only
hours to perform. The assay is easy, cheap and reliable,
and can be used at every cell passage. |
|
| *The PCR process is covered
by patents owned by Hoffmann-La Roche, Inc. Use of
the PCR process requires a license. |
|
| MycoScanTM assay features: |
High sensitivity - Will detect even small amounts of mycoplasma DNA
in your sample.
Specificity -
MycoScanTM is specific
for more than 25 "typical" mycoplasma species.
Reliable - The kit contains internal
control DNA and positive control DNA to optimize re-producibility
and minimize operator-dependent variability of results.
Rapid - The whole process can be
completed in about 5 hours.
Universal - One protocol using standard lab equipment detects
all mycoplasma and acho- leplasma infections that
commonly contaminate cell cultures.
Convenient - The kit contains optimized PCR reagents that conveniently
allow testing to be performed periodically or routinely
for quality control.
Easy to use - Allows for direct testing of culture stocks: elimination
of antibiotics or special growth conditions are not
required for testing.
Samples are easily
prepared - Templates for PCR analysis are
prepared by boiling the supernatant of cell cultures
or other samples.
Results can be clearly
determined - A clear and easily interpretable
result is obtained after PCR reactions. |
|
| Ordering
information |
| Please
send your request to: operation@hdbiosciences.com or call us at (+86)-21-58558330 |
|
| Description |
Catalog No. |
Unit |
| MycoScanTM |
HD01-0125 |
20 Tests |
| MycoScanTM Midi kit |
HD01-0150 |
30 Tests |
| MycoScanTM Maxi Kit |
HD01-0110 |
50 Tests |
|
|
|
| Testing
principles |
| The HD Biosciences
MycoScanTM assay
incorporates the highly-sensitive PCR technology as
its method for detecting contaminating mycoplasma.
The rRNA gene sequences of mycoplasma are well conserved.
The lengths and sequences of the spacer region in
the rRNA operon (in our case, the region between 16S
and 23S gene) differ from species to species. The
detection procedure utilizing PCR process with following
two steps: |
|
Step
1. Amplify the spacer region using two primers
(F1 and R1) on the DNA encoding rRNA of 16S and 23S.
Step 2. Perform Nested PCR using
two primers (F2 and R2). |
|
| This system
does not allow the amplification of DNA originated
from other sources, such as cultured cells, which
affect the detection result. Amplification of gene
sequence with PCR using these primer pairs enhances
not only the sensitivity but also the specificity
of detection. |
|
|
| Background |
The presence of mycoplasma
- a unique group of microorganisms that fall in the
category between bacteria and viruses - in cell cultures
can have a dramatic impact on the growth and metabolism
of the cells, and a knock-on effect on the yield –
and possibly the activity - of the recombinant protein.
Conventional techniques used to detect mycoplasma
involve culturing samples on selective media, which
needs at least a week to obtain results. By performing
PCR with our primer pairs, detection results are obtained
in a few hours. As the presence of contaminant mycoplasma
can be easily detected by only verifying the bands
of amplified DNA fragments in electrophoresis, there
is no need to prepare probes labeled with radioisotope,
etc.
The MycoScanTM assay
can detect many species of mycoplasma, including M.
fermentans, M. hyorhinis, M. arginini, M. orale, M.
hominis, M. arthritidis, M. hyopneumoniae and Acholeplasma
laidlawii, which account for more than 96% of
cell culture infections. |
|
| Need
elimination service & "clean" cells? |
| HD Biosciences can
also provide mycoplasma elimination services for you.
We have commonly used cell lines without any mycoplasma
contamination, which can be delivered as per as your
request. |
|
| For research use only. Not for use in diagnostic procedures. |
|
| For the
service, please contact us at kit@hdbiosciences.com,
or by phone: (+86) 21-58558330, fax: (+86) 21-58558011 |
