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Kinase Assay

HD Biosciences offers high quality, time efficient and cost-effective kinase solution for assay development, high throughput screening (HTS) and selectivity profiling.
Biochemical assays
Formats: Caliper, Kinase Glo Plus, Radiometric, IMAP, ADP-Quest, Z-Lyte, etc.
Assay development includes enzyme kinetic analysis, enzyme titration and assay validation.
Assay development is applicable for a broad range of kinases involved in MAPK pathway, cell cycle, NF-kB pathway, AKT/PKB pathway, JAK/STAT, membrane receptor, etc.
Figure 1: Results from various protein kinase biochemical assays developed at HDB
(A) Establishment of initial velocity for the determination of kinase A ATP Km.
(B) ATP Km determination for kinase A using ADP Quest format.
(C) Kinase profiling-IC50 determination using Kinase Glo Plus. Curve fitting was performed using GraphPad      Prism 5 software.
Kinase Selectivity Profiling Services
Profiling format: Caliper, Kinase Glo Plus, IMAP or Z'Lyte
The diversified panels of 23, 59 or more kinases for profiling services.
Single dose measurement and IC50 determination
Single compound test and library screening Data turnaround time: 1-2 weeks

Figure 2 Kinase selectivity profile of compound A against panel of 30 kinases.
High Throughput screening
Screening format: Caliper, Kinase Glo Plus, Radiometric, IMAP, Z'Lyte, etc
High throughput screening for biochemistry assays
Capable of generating dose curves for >5,000 compounds per week
High throughput screening for cell based assays
Capable of generating dose curve for >1000 compounds per week
Data turnaround time
1-2 weeks
384-well format is routinely used for high throughput screening
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HD Biosciences (China) Co.,Ltd
590 Ruiqing Road
Zhangjiang East Campus, Pudong, Shanghai 201201,
P.R.China
Tel: (+86) 21-51163700
Fax: (+86) 21-51163766
operation@hdbiosciences.com