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Kinase Assay

HD Biosciences offers high quality, time efficient and cost-effective kinase solution for assay development, high throughput screening (HTS) and selectivity profiling.
Biochemical assays
Formats: Caliper, Kinase Glo Plus, Radiometric, IMAP, ADP-Quest, Z-Lyte, etc.
Assay development includes enzyme kinetic analysis, enzyme titration and assay validation.
Assay development is applicable for a broad range of kinases involved in MAPK pathway, cell cycle, NF-kB pathway, AKT/PKB pathway, JAK/STAT, membrane receptor, etc.
Figure 1: Results from various protein kinase biochemical assays developed at HDB
(A) Establishment of initial velocity for the determination of kinase A ATP Km.
(B) ATP Km determination for kinase A using ADP Quest format.
(C) Kinase profiling-IC50 determination using Kinase Glo Plus. Curve fitting was performed using GraphPad      Prism 5 software.
Kinase Selectivity Profiling Services
Profiling format: Caliper, Kinase Glo Plus, ADP Glo
The diversified panel for profiling services.
Single dose measurement and IC50 determination
Single compound test and library screening Data turnaround time: 1-2 weeks

Figure 2 Kinase selectivity profile of compound A against panel of 30 kinases.
High Throughput screening
Screening format: Caliper, Kinase Glo Plus, ADP Glo, Radiometric, Z'Lyte, etc
High throughput screening for biochemistry assays
Capable of generating dose curves for >5,000 compounds per week
High throughput screening for cell based assays
Capable of generating dose curve for >1000 compounds per week
Data turnaround time
1-2 weeks
384-well format is routinely used for high throughput screening
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HD Biosciences (China) Co.,Ltd
590 Ruiqing Road
Zhangjiang East Campus, Pudong, Shanghai 201201,
P.R.China
Tel: (+86) 21-51163700
Fax: (+86) 21-51163766
operation@hdbiosciences.com