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Imaging and High Content Assays/Screening (HCA/HCS)

HDB offers compound screening and profiling services to assist our clients for lead identification and optimization. We focus our efforts on lead selection and assessment to gear at providing clients with reliable and accurate information through scientific procedures. Panels of profiling assays including biochemical assays and cell-based functional assays (i.e. in various target classes such as GPCRs, enzymes, ion channels and transporters), in vitro ADMET assays and pathway-based cellular phenotypic assays as well as high-content assays offer an integrated tool for clients to better characterize and optimize the pharmacology and toxicity profiles of their lead compounds.

Genotoxicity and cytotoxicity (micronucleus test; tube formation; caspase activation)
Cell signaling and transcription factors
Cell proliferation and cell cycles
Apoptosis and viability
Kinase translocation and GPCRs redistribution
Morphological and phenotypic changes
Imaging Platform
Automated cell imaging systems, ImageXpressMICRO (Molecular Devices)
Powerful analysis software: MetaXpress Software and Assay Application Modules
AcuityXpress Cellular Informatics Software
Advanced large scale data storage and IT support
Service Features:
Subcellular localization and quantitation of the cellular targets
Multiplexing capabilities: multiple data points from a single assay well
Parallel analysis of functional activity and cytotoxicity in one assay well
High sensitivity (nuclei staining allows for normalization of cellular signals against cell number)
Measurement of individual cell responses in the heterogeneous cell populations
Application of drug to live cells may indicate side-effects at the early stages of drug development
Cell based assays provide a biologically relevant assessment of compound functional activity
Robust and cost-effective assays
Minimum compound requirement (96 or 384-well plate format)
Compound Profiling
Pharmacology profiling has become an invaluable tool for lead compound selection, optimization and candidate prioritization. Analysis of profile data identifies off-target interactions that can help predict potential side-effects, efficacy and safety of compounds under development before they progress to the more costly stages of preclinical and clinical trials. HDB offers comprehensive profiling services to fit our clients¡¯ drug discovery & development needs.
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HD Biosciences (China) Co.,Ltd
590 Ruiqing Road
Zhangjiang East Campus, Pudong, Shanghai 201201,
P.R.China
Tel: (+86) 21-51163700
Fax: (+86) 21-51163766
operation@hdbiosciences.com