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Cell Lines Collection
HD Biosciences' proprietary technologies enable us to provide high-quality recombinant cell lines for various functional assays and for high throughput drug screening. Our cell lines with over expressed G-protein coupled receptor, ion channels or other drug targets provide solutions for drug discovery. The functional assays can be transcriptional readout (reporter gene assays) and/or intracellular calcium flux detection for GPCRs, and membrane potential dyes or calcium influx for certain ion channels. All HDB cell lines have been well-characterized and show stability in assays for at least 30 passages. (The Sample of Cell Line Product Description). All cell lines are available frozen at 2 x 106 cells/ml/vials and have been tested negative for mycoplasma.
Examples of Agonist/Antagonist Responsive Curves for GPCR
Examples of Agonist/Antagonist Responsive Curves for Ion Channel
To see our Cell line list
 Family
 Receptor
 Cell Line
 Assay
Adrenergic
¦Á1AR
HEK293/¦Á1AR
calcium/cAMP
¦Á1BR
HEK293/¦Á1BR
calcium/cAMP
¦Á1DR
HEK293/¦Á1DR
calcium/cAMP
¦Á2AR
HEK293/¦Á2AR
calcium/cAMP
¦Á2BR
HEK293/¦Á2BR
calcium/cAMP
¦Á2CR
HEK293/¦Á2CR
calcium/cAMP
¦Â2R
CHO or HEK293/CRE/d2EGFP/¦Â2R
EGFP/cAMP
¦Â3R
HEK293/¦Â3R
calcium/cAMP
Calcitonin
CTR
HEK293/CTR/CRE/d2EGFP
EGFP/cAMP
Cannabinoid
CB1
HEK293/CB1/G15
calcium/cAMP
CB2
HEK293/CB2/G15
calcium/cAMP
Cholecystokinin
CCKAR
HEK293/CCKAR
calcium
CCKBR
HEK293/CCKBR
calcium
Corticotrophin releasing factor
CRHR1
HEK293/CRHR1
cAMP
CRHR2
HEK293/CRHR2
cAMP
Dopamine
D1R
HEK293/D1R/CRE/¦Â-lactamase
¦Â-lactamase/cAMP
D2R
HEK293/D2R/Ga15
calcium
D5R
HEK293/D5R/CRE/¦Â-lactamase
¦Â-lactamase/cAMP
Galanin
GALR1
HEK293/GALR1/Ga15
calcium
GALR3
HEK293/GALR3
calcium
Glucagon
GCGR
HEK293/GCGR
cAMP
Glutamate
mGluR 1
HEK293/mGluR1
calcium
mGluR 2
HEK293/mGluR2/G16
calcium
mGluR 3
HEK293/mGluR3/EAAC1/G16
calcium
mGluR 4
HEK293/mGluR4/G15
calcium
mGluR 5
HEK293/mGluR5
calcium
rat mGluR 5
HEK293/rmGluR5
calcium
mGluR 6
HEK293/mGluR6/G15
calcium
mGluR 7
HEK293/mGluR7/EAAC1/G16
calcium
mGluR 8
HEK293/mGluR8/EAAC1/G16
calcium
Gonadotrophin-releasing hormone
GnRHR
HEK293/GnRHR/NFAT/¦Â-lactamase
¦Â-lactamase/calcium
Free fatty acid
GPR40
HEK293/GPR40/NFAT/¦Â-lactamase
¦Â-lactamase/calcium
GPR120
HEK293/GPR120/NFAT/¦Â-lactamase
¦Â-lactamase/calcium
Histamine
H1R
HEK293/H1R/NFAT/¦Â-lactamase
¦Â-lactamase/calcium
H2R
HEK293/H2R/CRE/¦Â-lactamase
¦Â-lactamase/cAMP
HEK293/H2R/CRE/luciferase
luciferase/cAMP
H3R
HEK293/H3R/Ga15
cAMP/calcium
HEK293/H3R/CRE/luciferase
luciferase/cAMP
H4R
HEK293/H4R/Ga16
cAMP/calcium
5-Hydroxytryptamine
5HT2CR
CHO/5HT2CR
cAMP/calcium
5HT7R
CHO/5HT7R/CRE/bla
¦Â-lactamase/cAMP
Melanocortin
MC1R
HEK293/MC1R/CRE/luciferase
luciferase
MC2R
HEK293/MC2R/CRE/luciferase
luciferase
MC3R
HEK293/MC3R/CRE/luciferase
luciferase
MC4R
HEK293/MC4R/CRE/d2EGFP
EGFP/cAMP
Melanin-concentration hormone
MCHR1
HEK293/MCHR1
calcium/cAMP
MCHR2
HEK293/MCHR2
calcium
Melatonin
MTNR1B
HEK293/MTNR1B
calcium
Muscarinic
M1R
HEK293/M1R
calcium
M2R
HEK293/M2R
calcium
Neuropeptide
NPYR1
HEK293/NPYR1/Ga15
calcium
NPYR2
HEK293/NPYR2/Ga15
calcium
Parathyroid hormone
PTHR1
HEK293/PTHR1/CRE/d2EGFP
EGFP/cAMP
HEK293/PTHR1/CRE/¦Â-lactamase
¦Â-lactamase/cAMP
PTHR2
HEK293/PTHR2/CRE/¦Â-lactamase
¦Â-lactamase/cAMP
Platelet activating factor
PAFR
HEK293/PAFR/NFAT/¦Â-lactamase
¦Â-lactamase/calcium
Substance P
SPR
HEK293/SPR
calcium
Orphan GPCRs
GPR7
HEK293/GPR7/G15
cAMP/calcium
GPR8
HEK293/GPR8/G15
cAMP/calcium
Tumor Cell Lines
Cell Line
Cell line
HEK293
SH-SY5Y
HEK293-T
BHK-21
7721 Cell
MCF-7
CHO-K1
Jurkat
NIH-3T3
ACHN
A549
HOP-92
3T3-L1
OVCAR-5
GP-293
SW620
Flp-In Tres 293
LoVo
CHO-T
HCC1937
COS-7
PC-3
HeLa
HT-1080
MDA-231
FAO
CaCo-2
For functional assay ion channel cell lines, please contact us for the details.
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HD Biosciences (China) Co.,Ltd
590 Ruiqing Road
Zhangjiang East Campus, Pudong, Shanghai 201201,
P.R.China
Tel: (+86) 21-51163700
Fax: (+86) 21-51163766
operation@hdbiosciences.com