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CRISPR/Cas9 Technology for Genome Editing
CRISPR/Cas9 genome editing system becomes a powerful tool to target and modify genomic sequence, allowing a previously unattainable level of efficacy, specificity and efficiency. This system uses non-coding RNAs to guide the Cas9 nuclease to generate sequence-specific DNA cleavage of target loci across the genome. HD Biosciences has fully implemented this technology platform to offer CRISPR/Cas9 based gene knock-in or
knock-out to support gene function studies and target validation efforts.
CRISPR/Cas9 Applications
Specific gene knock-out
Precise genome editing, such as site-mutation knock-in or add reporter gene tag
Rapid generation of genetically engineered mice
Genome-wide scale knock-out for screening
Key Advantages of CRISPR/Cas9
Simple and fast two-component system with high efficiency and low cost
Permanently turn off genes at the DNA level, clean background
Offers access to non-coding regions of the genome
HDB CRISPR/Cas9 Services
sgRNA design, plasmid construction, and validation
Knock-out of single or multiple target sequences
Site-mutation knock-in and gene tag knock-in
Isogenic cell line generation with gene knock-out/knock-in
Genome-wide CRISPR/Cas9 library screen
Case study 1: Knock-out of EGFP expression
U2OS cells with stable EGFP expression(U2OS_EGFP) were transfected with lentiCRISPR vectors containing Cas9 and either scramble sgRNA or sgRNA specifically targeting EGFP.
Case study 2: Target X Knock-out - CRISPR plasmid transfection
Tumor cells were transfected with vectors containing Cas9 and either scramble sgRNA or sgRNA specifically targeting Target X in order to generate isogenic Target X KO cell lines.
Case study 3: Target Y Knock-out - LentiCRISPR transduction
Tumor cells were transduced with LentiCRISPR vector containing Cas9 and either scramble sgRNA or sgRNA specifically targeting Target Y in order to generate isogenic Target Y KO
cell lines.
Flow chart for isogenic cell line generation
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HD Biosciences (China) Co.,Ltd
590 Ruiqing Road
Zhangjiang East Campus, Pudong, Shanghai 201201,
P.R.China
Tel: +86 (21) 5116 3700
Fax: +86 (21) 5116 3766
info@hdbiosciences.com
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