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Biologics Discovery
As a class of medicine, biologics are a powerful tool that can often treat diseases that cannot be treated with traditional approaches. Biologics Discovery Program at HDB has built core competency with comprehensive platforms and experienced technical team over the last a few years which provides quality and efficient services to our clients in the world. Currently we provide the following services
1 Therapeutic antibody discovery
1 Monoclonal antibody generation
1 Immunogenicity test
1 Other platforms
Therapeutic antibody discovery
1 scFv/Fab phage display vector construction and optimization
1 scFv/Fab human antibody phage display library construction and characterization
每 Start from PBMC, B cells, or commercial RNA
每 10E5 or above diversity for light chain
每 10E8 or above diversity for heavy chain
每 10E9 to 10E11 diversity for scFv/Fab
每 >70% functionality for library clones
每 Duplication less than 1%
1 Library panning, target antibody identification and characterization
每 Phage ELISA
每 Filter lift
每 Competitive ELISA
每 BIAcore affinity and kinetic analysis
1 Antibody maturation and engineering
每 Chain shuffling
每 CDR walking
每 Fc engineering
1 Antibody expression and purification
1 Antibody functional assays
每 Blocking or activating target function
每 Apoptosis
每 Effector function: ADCC; CDC.
Monoclonal antibody generation
1 Monoclonal antibody generation
1 Resubcloning and expression in CHO cells
1 Binding/neutralization assays
1 Antibody affinity analysis (BIAcore)
1 Epitope mapping
Immunogenicity test
Three major steps
1 Screening assays: minimize false negative
1 Confirmation assays: antibody positivity in patients with or without use of excess drug
1 Neutralization assays: Effect of antibodies on the efficacy of the drug in functional assays
Format of assays
1 ELISA, Western blot, BIAcore
1 Ligand binding/competition assays
1 Neutralization assays (cell based): cytokine release, calcium release, protein phosphorylation, cell
proliferation or apoptosis
Other platforms
Humanization of mouse monoclonal antibodies
1 Chimericl antibody generation
1 CDR grafting
1 FW residue resurfacing
 
Vaccine development
1 Peptide vaccines: overlapping peptide library, CTL generation and characterization, DC generation and
characterization, antigen presentation
1 DNA vaccines: vector constructions, mouse immunization, xenograft, growth and survival evaluation
1 Protein/cell vaccines
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HD Biosciences (China) Co.,Ltd
590 Ruiqing Road
Zhangjiang East Campus, Pudong, Shanghai 201201,
P.R.China
Tel: (+86) 21-51163700
Fax: (+86) 21-51163766
operation@hdbiosciences.com