HD Biosciences specializes in functional assay development and services. Functional assays play a pivotal role in the decision to move test compounds from in vitro to in vivo pharmacology study for a variety of drug targets. The evaluation of biological effects of hit or lead compounds on target-mediated cellular signal transduction and other primary events by changes in cellular behaviour is a core part of our in vitro assays. HDB also performs comprehensive enzyme and biochemical assays to complement cell-based assays and provides full assay solutions for your discovery needs.
Comprehensive Assay Formats for Diversified Target Groups
Target
Assay Format
Remark
GPCR
CRE-Reporter gene (Luc, Blac, eGFP)
For Gs-coupled GPCR
NFAT- Reporter gene (Luc, Blac, eGFP)
For Gs-coupled GPCR
Calcium assay (Fluo-3, Aequorin)
For Gs-coupled GPCR
G15/G16 + Calcium
For Gs-coupled GPCR & Orphan GPCR
cAMP assay
For Gs & Gi coupled GPCR
Ion Channel
Calcium influx detection
Membrane potential assay
Electrophysiology assay
all channels
Dye-uptake assay
For channels which form a hole on the cell membrane
Kinase
Z-lyte assay
FRET
Kinase Glo assay
Luminescence
IMAP assay
Fluorescence Polarization
ADP Quest
Fluorescence
Cell Based assay
transient or stable cells
Antiviruses
Quantitative RT-PCR
For viral integrase assay
HIV/HCV replication assay
Report gene system
Quantitative ELISA
PCR/RT-PCR
MTT assay
For cell proliferation and cytotoxicity
Others
NO production assay
cGMP detection assay
Chemo-sensitization assay
Large scale transfection
Suspension cell lines
Knock-out cell line generation with RNAi technology
