| HDB focuses
on functional assays, which play a pivotal role in
the decision to move from in-vitro to in-vivo pharmacology
for varieties of drug targets. The evaluation of the
effects of hit/lead compounds on target-mediated cellular
signal transduction and other primary events into
changes in cellular behaviors is a core part of our
assays. |
|
| Assay
development for GPCRs: Our state-of-the-art
GPCR* technologies enable us to perform almost all
types of assays for different classes of G-protein
coupled receptors, including Gq, Gs, Gi, and orphan
receptors. The assay platform for GPCR includes: |
 |
Cell-based
functional assays |
|
Reporter genes (CRE, NFAT, AP-1) |
|
Real-time calcium flux (FLIPR/Flexstation) |
|
Gq,
Gs and Gi-coupled receptor assay capability |
|
Orphan
receptor assays with promiscuous G-proteins (Galpha15/16) |
|
| Assay
development for ion channels, various kinases, antiviral,
apoptosis, and other molecular targets |
| All of
our assays are HTS compatible. The assays can be used
in primary or secondary screening. |
|
| The
assays are available in the following formats: |
|
Transcriptional
readout-based functional GPCR assays (fluorescence,
bioluminescence) |
|
Assays to detect intracellular calcium flux with calcium
sensitive dyes |
|
Tyrosine kinase assays, nuclear receptors (fluorescence
polarization, Enzyme Fragment Complementation) |
|
Voltage-sensitive
dyes and calcium dyes for ion channels |
|
Cell
viability assays (MTS) |
|
Annexin
V based apoptosis assay |
|
| Currently,
HDB has a
GPCR target bank with more than a hundred GPCRs and a cell line bank
available for immediate assays and screening. HDB
also has over fifty different assays for various targets.
All of the targets and assays are made available to
our partners and customers. |
|
unstim cell d2-EGFP
stim cell d2-EGFP |
|
| Our
reputation in the biotech industry proves that we
can provide services with quality you can count on. HDB provides the ultimate solution for your assay
development. HDB has successfully entered several
collaboration and service agreements with leading
pharmaceutical and biotech companies worldwide. |
|
| Please
contact us at operation@hdbiosciences.com to get a detailed quote for our services. |
