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Anti-bacteria Program
With an experienced team, fully equipped BSL-2 microbiology labs, and ABSL-2 animal facility, HDB has built up comprehensive capabilities and capacities to support anti-bacterial drug discovery. The company has the most extensive collections of characterized bacterial strains, varieties of assays and matching screening capability. Mechanism-of-action (MOA) studies are also part of our service, which includes kinetic study,
transcription & translation assay, nephrotoxicity assay, etc.
Our anti-bacterial service includes:
Antibacterial susceptibility testing:
Transcription and translation assay
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Minimum inhibition concentration
(RNA and protein synthesis):
(MIC) screening of compound library
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E. coli S30
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MIC / MBC / MIC50 / MIC90 testing
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E. coli erythromycin-resistance S30
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S. aureus S30
Kinetics study:
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Time-kill study
Bacterial lysis assay (Cell membrane /
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Post-antibiotic-effect (PAE) study
cell wall structures):
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Bacterial membrane depolarization
Synergy test:
assay
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Checkerboard assay
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Time-kill or E-test methods
Target specific assays:
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E. coli and S. aureus gyrase assay
Resistance test:
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E. coli and S. aureus topo IV study
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Frequency-of-resistance (FOR) assay
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tRNA-aa ligation assay
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Resistance mutant gene identification
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Other assays
Macromolecule synthesis pathway
Toxicity assay:
radiolabeling:
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Cell-based nephrotoxicity assay
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DNA, RNA, protein, phospholipid,
peptidoglycan, and polysaccharide
synthesis in E. coli, S. aureus, P.
aeruginosa and other bacteria strains.
Bacterial Strain Collection:
HDB’s bank of bacteria strains contains over 600 clinical isolates and a rich collection of standard ATCC strains. This unique collection can assist you to profile your lead compounds and candidates.
All strains are validated via:
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16S rRNA amplification and AST using Vitek 2
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Drug resistant gene identification through multi-plex PCR and sequencing
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Whole genome sequencing
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HD Biosciences (China) Co.,Ltd
590 Ruiqing Road
Zhangjiang East Campus, Pudong, Shanghai 201201,
P.R.China
Tel: +86 (21) 5116 3700
Fax: +86 (21) 5116 3766
info@hdbiosciences.com
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